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KMID : 0358219960230030293
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Korean Journal of Fertility and Sterility
1996 Volume.23 No. 3 p.293 ~ p.302
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Effect of Electric Stimulation(anion pad) on the Maturation of Follicular and the Cleavage of Fertilized Embryos of the Mouse
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배인하
박원/최성미/김문규
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Abstract
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In the present study, mouse follicular oocytes and 2-cell embryos (late -zygote stage embryos included) were cultured on the electric pad for electric stimulation in the culture incubator. In addition, follicular oocytes and embryos were tested
for
maturation and development under higher temperature condition (39℃). Mouse follicular oocyte maturation were not affected by anion electric stimulation and there is no significant difference in GBVD and MI between the control and experiment
group
after
4hr culture.
In the embryo culture, it was found that more morula and blastocyst were found in the electric stimulation group rather than the control (96hr). This may seem to the caused with cytoplasmic Ca2+ transient rise by electric stimulation (anion pad).
On the
other hand higher temperature incubation (39℃) on the anion pad caused all the embryos degenerated within 12h~24hr culture. This was quite different from large animal embryos (bovine, pig, sheep), in which beneficial effect of high temperature
incubation for oocyte maturation and embryo development were found.
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